If I isolate/enrich lysosomes from mammalian cells through either differential centrifugation or using commercial kits, what is the best method of storage to preserve enzymatic activity? Is there a specific buffer that would be the most appropriate? Organelles should be intact at this point so would slow freezing as with cells be more appropriate than snap freezing with liquid nitrogen?
NB: structural integrity of lysosomes is less important than enzymatic activity.
Thank you,
Sam
Use rapid-freezing cytochemistry. Article Rapid-freezing cytochemistry: Preservation of tubular lysoso...
Thank you for your suggestion Maurice Ekpenyong . Although I’m hoping to look at enzyme activity rather than any cytochemistry. Reading the paper, it sounds like DMSO and snap freezing with LN2 similar to Freezing live cells would be enough. I may have to run a comparison.
best wishes.
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