Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

How to prevent cancer spheroid aggregation in culture?

I'm trying to grow PDX-derived cells from a SCCOHT model and I need them to survive short-term (7-14days) ex vivo After tumor digestion I platted 7,000,000 cells in a 10cm ULA dish In the...

26 February 2020 1,401 4 View

How could I extract residues pesticides fron sheep wool?

Hi, I want to analyse residues of Cipermetrine, ethion, amitraz, diazinon and other pesticides extracted from wool, by using HPLC/MSMS system. I would like to know the best way to extract the...

15 August 2019 9,350 2 View

I made a saline solution of NaCl 0,9% andajusted the pH to 11 with NaOH. After 3 months I read the Ph and it had come down to 8. Does anyone know why?

I've also made a saline solution of NaCl 0,9% pH 9 and after 3 months it came down to pH 5. I don't understand why the pH came so drastcally down. I tested for any contamination and the results...

17 December 2018 8,793 3 View

How to release a protein (80 kDa) from a polymeric nanoparticle?

10 November 2018 6,963 3 View

I would like to talk to someone who extracts RNA from ganglion optics in crustaceans?

I'm trying to extract the RNA from the shrimp optical ganglion.

18 October 2018 2,959 1 View

Can you help me understand an inconsistency between copy-number variation of a gene and its expression?

Hi fellow scientists, I am researching a gene in Enterococcus faecalis, classified in two groups: 1) Penicillin-sensitive/ampicillin-sensitive (Control) and; 2)...

17 September 2018 9,809 4 View

Why the current backward is higher than the forward?

Hi, does anyone knows why my CV in KOH 1M and EtOH 1M of Palladium in graphene oxide the current forward is lower than the current backward? It should be the opposit, or am i wrong? In the...

05 April 2018 1,687 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View