You should check the sensitivity and range of your kit. Otherwise, many kits are especially designed for rat's hormones. For testosterone and insulin I use the Alpco kit, for corticosterone I use crystalchem.

Unlike peptide or protein hormones, steroids structurally similar. Hence, It is possible to measure Testosterone using a human ELISA kit.

There are many kits available on the market which detects human/mouse/rat and others; the reason for mention cross-reactivity, sensitivity, specificity because of the matrix interference (Serum/plasma/urine/cell culture supernatant) between inter/intra-species.

And I am not clear about other "Male hormones"; are you trying to ask about dehydroepiandrosterone?

Dear dr. Ali,

I think this is depends on kit manufacturing and standard of company( procedure) preparation.