How much sample size is necessary for the cross sectional study done in school/college level students about their smoking and drinking behaviors?

26 September 2020 8,101 7 View

How do I interpret these western blot results?

Hi! I got this western blot of the heat shock protein hsp70, and I need to interpret the result. The western is made on 2 individuals A and I and is shocked 5 minutes and 15 minutes, plus there is...

17 July 2020 167 5 View

How to use AutoDock Tools for Virtual Ligand Screening?

I downloaded the AA tranche from ZINC as a pdbqt.gz file. Following decompression of the file, can I go straight to docking using Raccoon or do I need to upload to ADT and add charges, all...

07 May 2020 3,704 1 View

I am conducting an ethnography with mixed method approach, what would be the best sequential order: Quali to Quanti or vice-versa?

I'm doing an insider ethnography and using mixed method approach among disaster survivors resilience. As I belong to the same population pool as that of participants, it is said that ethnography...

19 March 2020 7,721 5 View

Is Driscoll-Kraay standard error model approach able to handle non-stationary panel data sets?

Please, I'd like to know whether Driscoll-Kraay standard error model approach (Stata command XTSCC) able to handle non-stationary I(1) panel data sets? In other words, is this a requirement for...

17 March 2020 944 6 View

Has anybody used the Elisa kits from Elisa Genie, LifeSpan BioSciences, Aviva Systems Biology, Abbexa and Antiboides-online?

I am looking for sandwich Elisa kits for human CTNND1, and I have found the kits from the above companies. However, our group are not familiar with these companies, and I cannot find publications...

02 February 2020 6,131 1 View

How to build a TiN cluster model for ab initio simulation?

Hi, There are many journals related to simulation that used Silicon cluster as the substrate model. I have also seen SiO2 cluster model on the research journal. However i can't seem to find any...

07 November 2019 8,171 1 View

Troubleshooting beta galactose staining protocol?

Hello all! I have som trouble staining beta galactose in my MC3T3 cells. Here is my protocol: - Cells stressed with H202 treatment. - washed twice in PBS - fixated in 4% paraformaldehyde 5min...

10 July 2019 9,207 4 View

Rules for paired sample t-test with samples from cell culture?

My experiment is isolating primary cells from biopsy of different patients. And then to culture the primary cells in two conditions, one is the negative control, and the experimental set-up is...

06 July 2019 6,194 5 View

Suitable Job Stress Instruments?

Currently, I'm doing my study on the emotional intelligence and job stress among the non-academic staff. Can i know the suitable job stress instruments suitable to use?

24 April 2019 6,247 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View