Estimate Beta is .00, yet it's significant?

Hi everyone, I've run an analysis of a latent 3-way interaction in Mplus (Y on XWZ). The estimate for this path is 0.000. S.E. is 0.000. Est./S.E. is 2.034. Two tailed p-value is 0.042. So,...

08 December 2020 2,132 4 View

What is the double layer capacitance of a 1 sq-cm smooth gold electrode in 0.1N (0.05M) Sulfuric Acid?

Is there an empirical or theoretical standard value for this (at room temperature) and for different electrolyte concentrations? Is there a resource available that provides a chart of values for...

29 November 2020 6,937 2 View

How does the concentration of atmospheric oxygen near ground level vary with time?

Hello, I am interested in how the concentration of O2 varies over time close to ground level. I have been informed that the concentration can drop when an inversion layer is present in the local...

19 November 2020 2,328 3 View

How does one calculate the best volume of nickel gel to use in a protein purification?

We know the capacity of the gel and the total amount of protein in the bacterial lysate from a Bradford assay. What we do not know is what fraction of the total protein is the his-tagged protein...

15 November 2020 4,664 2 View

Final temperature of water leaving an ice bath through copper tubing?

I have water flowing at 4 GPM at 2.5 feet per second through 50ft of 0.375 in. outer diameter tubing with 0.014 in. wall thickness. The water flows through copper tubing into the 50ft coil...

01 November 2020 8,039 5 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

13 July 2020 1,773 3 View

Where to find predator:prey mass data (for terrestrial carnivores)?

I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds). An example of is...

16 June 2020 2,453 9 View

How to calculate the overlap between animal distributions (Shape or .shp file)?

I would like to calculate the overlap between the distributions of two sets of data (predator & prey). The prey set has got about 250 species, the predator set approximately 5000. For each...

21 May 2020 6,726 3 View

Who can assist with an ingredient design of a Covid 19 Sars-2 Cov protein subunit and adjuvant vaccine?

18 May 2020 1,675 13 View

No title

23 February 2021 4,014 3 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does increasing antibiotic concentration also increase plasmid production in DH5a E.coli?

Dear all, I am propagating some plasmids with a human gene and an ampicillin resistance gene. My logic is that if ampicillin concentration will be increased, the concentration of plasmid (pSF)...

14 January 2021 779 3 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View

Which should be used for Anxiety : unselective beta blocker or B1 Blocker ?

which would be more effective in treatment or protection from Anxiety : unselective beta blocker as Propranolol or selective beta-1 adrenergic receptor antagonist as Bisoprolol ?

15 November 2020 7,994 2 View