How can I calculate the total TG content in the rat skeletal muscle using a commercially available kit (Sekisui)?

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Gordon L Warren

What specifically is your question? I have measured triglyceride (TG) content in rat and mouse muscles on a few occasions. Measuring TG content was the easy part. Extracting TG in preparation for measurement was the harder part.

Leila Baghersad

Thanks, Gordon for your reply.

Actually, I want to make sure that my calculation is correct.

Briefly, the final concentrations were normalized based on muscle weight and then multiplied by 2, because I separated extracted lipids in two vials equally. This is how I calculated lipid concentration.