Technical protocol to proteins extract from rat white adipose tissue?

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What are the pros and cons for mice versus rats?

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Martin Hofmeister

Dear Sylvie, the following paper may help you: Diaz Marin R, Crespo-Garcia S, Wilson AM, Sapieha P. RELi protocol: Optimization for protein extraction from white, brown and beige adipose tissues. MethodsX. 2019;6:918-28. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500908/pdf/main.pdf

Best wishes from Germany, Martin

Sylvie Grall

Thanks Martin! It's exactly THE paper we found! It seems there's no other technics in case of WAT extraction difficulties! Regards, Sylvie

Quanzhi Li

Following kit will work well:

https://inventbiotech.com/products/minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes?_pos=1&_sid=eedbea502&_ss=r