What is the best approach to induction of anemia in experimental rats?
Ayodeji Odukoya A carcinogenic drug Benzo (a) Pyrene induce cancer and this will lead the anemia in mouse model. This drug is total carcinogenic mean this initiate, proliferate and development the drug. After cancer development cancer lead to anemia (loss of erythrocytes).
We can also induce anemia in rats by using. We induced anemia in rats using phenylhydrazine (PHZ) and injected either saline or ZnSO4 solution.
I am sharing a article highlighted with yellow color.
Intraperitoneal administration of Cyclophosphamide, phenyl-hydrazine or Adenine in a dosage per weight ratio are the most used chemical compounds in the induction of anaemia in wistar rats..
Kindly note that Oral administration, Dermal administration and Inhalation of this compounds could also results in damage to the Red Blood Cells and consequently Anemia.
Dear Ayodeji Odukoya,
To induce anemia experimentally in rats to provide a hematocrit of 30 to 35% and provide data about the physiologic response to anemia during induction and after it is established, two methods were selected: the application of repetitive doses of phenylhydrazine and bleeding. Blood sample collection was carried out at various times, and hematologic profiles and osmotic resistance were evaluated. The morphologic features of cells and distinct organs also were examined. Results indicated similar decrease of hematocrit and hemoglobin concentration for the two experimental groups, although the reticulocyte response was higher in rats treated with phenylhydrazine, where the presence of young erythrocyte populations was linked to increases in osmotic resistance and glucose utilization. 2,3-Diphosphoglycerate was induced only during the recovery phase of the study and corresponded to a gradual response to hypoxia. Histologically, marked erythroid hyperplasia was found in the bone marrow, and extramedullary hematopoiesis was seen in the spleen and liver.
Once please go "Physiological response to experimentally induced anemia in rats: a comparative study" this will be helpful for your study.
We do not use wistar rats but we induced anemia in BL6 mice be feeding them from week 3 (crucial time point) on a low iron diet (
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