While reviewing my qPCR, I noticed that I had different melt peaks for samples run in triplicate. Is this something I should be worried about, or is this normal? I've uploaded a word doc showing graphs of a few of them for reference.
From which sourse did you make the DNA extraction? Is possible that during your DNA extraction, you co-extracted something substance that interfer which the qPCR (for exmaple in the DNA extraction from soil is the humic acid). Did you try to run your qPCR-product on the gel?
I had the same experience with melt peak. It happened when the quality and quantity of my samples varied. If you have extracted your samples with the method, the 260/280 or 230/260 rations are the same and quantity of template is adjusted, you should not see this issue.