Hi everyone.
I observed my staining and everything was okay and decided to continue the next day only to found my hoechst staining become hazy. Do you know why?
I mounted them using 1x H-1400-10 VECTASHIELD[R] HardSet.
As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...
26 February 2021 6,905 10 View
Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards
24 February 2021 9,690 1 View
I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...
23 February 2021 1,631 3 View
this is for my thesis project, so this is the title of my thesis, the effect of land use on hydrological components in Muar, Johor, Malaysia any ideas, guidance, and suggestions all? Thank You, :)
02 February 2021 265 5 View
I need to send my sample to outsource laboratory and how can i tell them that i want to run the FTIR for structural properties of protein? is there any differenece between FTIR for functional...
15 January 2021 8,992 1 View
10 January 2021 127 2 View
08 January 2021 920 16 View
I found that, there is a difference in pHPZC value when higher impregnation ratio of acid on adsorbent surface. Lower pHPZC value when higher ratio of acid impregnation and adsorption performance...
05 January 2021 7,224 3 View
I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...
05 January 2021 2,320 5 View
The current situation the virus become mutation, but there were information that zero percent case in Wuhan?
31 December 2020 2,457 4 View
03 March 2021 8,272 1 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View