Why did my hoechst staining become hazy after overnight in cold room?

Nur Izzah Farhana Binti Mohamad Azhar @Nur-Izzah-Farhana-Mohamad-Azhar

06 September 2022 0 8K Report

Hi everyone.

I observed my staining and everything was okay and decided to continue the next day only to found my hoechst staining become hazy. Do you know why?

I mounted them using 1x H-1400-10 VECTASHIELD[R] HardSet.

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Science method

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