It is not compatible with MS as buffer in the mobile phase for LC, but is it OK to have in sample? The concentration is around 70mM. Thanks.
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...
09 July 2024 7,883 0 View
Using magnetron sputtering with an alloy target composed of A:B:C in a 2:1:1 ratio, the resulting thin film composition is A:B:C=1:1:1. How can you achieve a A:B:C=2:1:1 composition? What are some...
24 June 2024 1,605 0 View
Given the energy level diagram of a ruby laser shown in the right figure, where \( R_{02} \) is the pumping rate, and \( \tau_2 \) and \( \tau_1 \) are the lifetimes of levels \( n_2 \) and \( n_1...
23 June 2024 4,855 0 View
Can the matter wave theory, which suggests that when matter waves resonate, the energy input from the outside is minimal, be used to solve all superconductivity problems? The matter wave theory,...
17 June 2024 7,957 0 View
till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...
12 June 2024 1,908 3 View
International Journal of Complexity in Applied Science and Technology(https://www.inderscience.com/jhome.php?jcode=ijcast) is a new journal. Can anyone tell me how to promote the new journal and...
09 June 2024 1,922 1 View
I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...
06 June 2024 941 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View