Hi all,
I am going to carry out some experiment about protein asorption on my titanium scaffolds. i want to use the SEM XPS in order to detect N picks, whic are most likely associated to amino-groups. The question is: what procedure do you suggest me i do in order to avoid contamination, or protein detachment before the xps analysis? Few papers reports to store samples in liquid nitrogen but the process itself is not that clear. If is possible, could you provide with a detalied protocol?
Thank you for your help,
Take the SEM XPS of your bare titanium scaffolds first. Depending on the fabrication method of your scaffolds you may find very small peaks for N in the XPS data. Then, run the protein adsorbed titanium scaffolds that provide significant peaks of N. Now you can simply calculate the amount of adsorbed protein on the scaffolds. During sampling and storing of the samples, it is better to avoid nitrogenous environments because the active site of the scaffolds may interact physically or chemically with N resulting over estimation of N can occur. So, other inert environments, such as liquid He or Ar medium can be useful.
I just add the importance of having enough replicates for such experiment to obtain reliable data with good precision and accuracy
Thank you Mr. Shavandi, I am going to match this analysis with BCA protein quantification.
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