Has anyone used X-gal solution from Millipore? can you share a protocol?

27 November 2019 1 7K Report

I have always used other reagents now I bought this one but I am not sure if there is an alternative protocol and I cannot find a lot of information online. Many thanks

Jorgaq Pata

https://www.goldbio.com/documents/1031/Blue%20White%20Screening%20of%20Bacterial%20Colonies%20using%20X-Gal%20and%20IPTG%20Plates.pdf

This protocol has been working fine, in your case, X Gal is already in solution, so you can just skip that step and add it to the agar supplemented media before pouring it on your plates.

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