I would like to ask the above question in context of intact protein characterization. Is it possible to detect DTT in positive mode using 0.1% formic acid gradient, and in which conditions?
To be good researcher, you should try to help yourself first and use the internet to your advantage. I spent only 1 min and found the paper below. It will be faster than waiting for someone on this board to help you out. I understand that you may not have access to the real paper depending on your internet account; however, at least it will help you how to do the right way.
Narong Chamkasem: Thank you for your valuable advice! I had already found that paper before I posted my question. However, that paper used NEGATIVE mode, while my question is about POSITIVE mode.
So, what is the issue to run in negative mode? The negative mode will give you better signal/noise ratio; therefore, you will get better sensitivity. If you need to monitor other peaks in positive mode, you can make a separate acquisition window for pos and neg mode.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins