Typical concentrations of glucose transporters in mouse brain?

I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...

21 December 2020 4,647 2 View

Is passage number of cell line crucial for developing leukemia in SCID mice?

Hi everyone, I am trying to develop leukemia in NOD SCID mice with BCR/ABL over expresssed cell line. But somehow the mice is not showing any signs of the disease. The passage number of cell line...

25 October 2020 5,614 3 View

I want to know the BET surface area of nano-rod shaped ZnO nano-particles?

Anyone if you know the BET surface area of nano-rod shaped ZnO nano-particles than please comment. The nano rod-shaped ZnO nanoparticles are synthesized by chemical precipitation method. If...

25 May 2020 8,856 1 View

What could be the reason for a fiber shape TiO2 nanowire besed dye sensitized solar not to work?

I am trying to fabricate a fiber shape TiO2 nanowire-based dye-sensitized solar cell on Ti wire. I use a hydrothermal route to grow the TiO2 nanowires. Then I absorb the nanowires with N719 dye. I...

15 December 2019 1,407 3 View

How to decompose a backbone curve into 10 elasto plastic parallel springs in abaqus?

Presently I am trying to implement the SSI technique on a pile on clay soil by attaching non-linear spring decomposed from the backbone curve for my master's project. can anyone help me how to...

15 October 2019 905 1 View

Can I use a sonicator to clean Ti wire?

I want to use Ti wire to make fiber shape solar cell. In literature they use an ultra-sonicator to clean Ti wire. Can I use a sonicator for this purpose as I do not have an ultra sonicator...

22 August 2019 5,066 9 View

Can i uncoat my gelatin from cell culture flask?

I have coated my culture flask with more concentrated gelatin than required accidentally. I haven't seeded my cells yet. Can i uncoat them and again coat them with desired concentration?

24 April 2019 529 1 View

Can i do immunofluorescence in cover slip for my HUVEC? If so, how?

I am going to do immunofluorescence from my cultured HUVEC. Before doing IF test i need to treat my cells with my test serum sample for 6 hours. I don't have chamber slide for this. So I am in...

08 April 2019 3,948 3 View

DNA analysis in glass microfluidic channel has showed a lot of sticking problem and eventually clog the channel. Any solution to reduce DNA sticking?

I am doing DNA separation in micro-fluidic channel network made of glass. There I see DNA sticking on glass channel wall that impedes DNA movements and eventually clog the channel. After few...

19 March 2019 6,839 3 View

How to preserve and process endocervical swab for cytokin analysis by flow cytometry?

I wanna analysis cytokines level in different stages of CIN and ca cervix both in HPV DNA positive and negative cases. We collected 1000microliter cervical swab by cyto brush for detection of HPV...

12 March 2019 2,628 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View