i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria? qnd if there is any software that can help me in doing this?
thanks in advance.
regards.
If it's in Excel, I'm assuming that you have a list of centroids as a function of mz. You can export this as a tab delimited spectral file and import this into mMass for a single spectrum, or if you have a series of (mz, centroid) files you can export these into tab-delimited spectra in a time series and import these into mzMine for LC/MS analysis. Both are open-source software. You may have to write some code to package the time series as a mzData or mzXML format before using mzMine.
Knowing which columns are in your Excel file and what mass spectrometer/analysis type they come from would be helpful prior to recommending anything. Assuming you need sequencing info, I agree with Luke V Schneider, but there are many other open source resources, it is just the matter of selecting the one that works with your data type.
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