Does anyone know the concentrations of the plate wash and dye release reagents in the Sircol 2.0 soluble collagen assay?

03 April 2024 1 7K Report

We used the wrong protocol and used all the plate wash and dye release reagents, but still have more of everything else. I wanted to see what to use so the rest of the kit doesn't go to waste.

Nick Chacos

Sircol is a sirius red based collagen assay. The wash buffer is most likely PBS or something very close. Release of dye from collagen is induced by treatment with a basic solution like 0.1 M NaOH. You can look on Google scholar for sirius red collagen assay and find many papers describing it.

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