What does it mean when I see 2 bands in SDS PAGE in my protein's molecular weight?

29 May 2024 1 4K Report

Hi, there are 2 bands in my protein's MW. What could be the reason for shifting the band from the original band?

My protein's molecular weight is 131 KDa and it is bound with a FAD cofactor. I have attached my gell which I ran after SEC column.

Mary Cristine Charlesworth

As you increase the load of a single protein in a gel, diffusion down into the gel occurs. You have just exceeded the capacity of the gel to “hold” hold that protein. For a single protein, using Coomassie stain, 0.2-0.5 ug is a good load to see a nice distinct band In a mini gel 10-15 lanes.

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