Understanding the susceptibility of small ruminants to artificial infection will provide insights into improved methods for the 'revival' and 'amplification' of frozen stabilates where mice might not be available or appropriate.
I am interested in the safety/toxicity of NTBC administration in different animals. There is a lot of data available relating to oral administration however limited data on intravenous administration.
04 June 2024 5,628 1 View
I cut 50 um sections of tissue in agarose and need to culture them for 14 days before fixing.The sections have great surface area when I first section them, but after 14 days they shrink to sizes...
28 May 2024 9,861 0 View
According to the journal article I've posted, the Sysmex DI-60 can detect the different morphologies of the RBCs. I want to find out if the Sysmex DI-60 can detect not only the morphology of the...
18 March 2024 8,225 3 View
The journal that was given to us stated that they recommended to use K2 EDTA blood test tubes to collect the blood samples of the patients.
06 February 2024 3,607 2 View
DALY is disability-adjusted life years QALY is quality-adjusted life years I will appreciate brief answers from colleague health economist and anyone who find it interesting working on these two...
16 October 2023 8,054 4 View
Please help!
24 May 2023 3,918 2 View
Hello, I'm working with primary mouse osteoblasts obtained through calvarial prep and both my coworker and I are having issues with a strange contamination that neither of us have seen before...
02 May 2023 2,016 3 View
I am involved in a research project related to soil organic carbon restauration. In littérature, it's recommended to sample at 0-10 ; 10-20 and 20-30 cm. I would like to know why these depths are...
05 April 2023 2,662 3 View
I am in need of answers as to knowing the limitations of blood gas analysis for our report.
07 August 2022 5,558 6 View
The use of directed acyclic graph (DAG) is gaining grounds in causal and prediction studies. Would you use DAG when deciding which covariates to adjust for, and why?
29 March 2022 8,294 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View