To remove paraffin from plant samples for microtome sectioning, first trim the paraffin block to expose the tissue, then use a rotary microtome to cut sections until the tissue is reached. Adjust the blade angle to about 10 degrees for optimal cutting. Finally, float the sections on warm water to flatten them before placing them onto slides for further processing.
Briefly , Immerse the plant tissue sections in xylene (or xylol) for about 10-15 minutes. Xylene is a strong solvent that dissolves paraffin efficiently. Sometimes, a second immersion might be necessary if the sections are particularly thick or if complete deparaffinization is needed...Note: Xylene is highly toxic and has strong fumes, so work in a well-ventilated area...
To remove paraffin from plant samples prepared for microtome sectioning, follow these steps:
1. Xylene Baths: Immerse sections in fresh xylene for 5-10 minutes, then transfer to a second xylene bath for another 5-10 minutes to dissolve paraffin completely.
2. Ethanol Series: Rinse sections in 100% ethanol for 2-5 minutes, followed by 95% and 70% ethanol for 2-5 minutes each to remove residual xylene.
3. Water Rinse: Finally, rinse in distilled water to remove ethanol and prepare the tissue for staining.
Use PPE and handle chemicals in a ventilated area.
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