I am a master's student in Korea. I have some questions while conducting a cell culture experiment on PDMS. 1. What should I do to allow HaCaT cells to grow on PDMS without O2 plasma treatment during cell culture? I know that many papers proceed with O2 plasma treatment to make PDMS hydrophilic, but I would like to experiment in a different way. As a result of the experiments so far, on the PDMS that has been autoclaved, they grow in clumps, not in the shape of HaCaT cells. The number of cells that can reach 80% confidence the next day was seeded. 2. we pour PDMS into the 24 well plate, mold it, and separate it to turn the autoclave. After that, we put PDMS back into the 24 well plate with tweezers and proceed with cell culture. Since it is manual to put PDMS with tweezers, media and cells flow next to PDMS in some wells, and cells grow on the bottom of the well plate. Would it be better to mold PDMS by building a wall instead of a flat surface to make it grow only on PDMS instead of on the well plate floor?
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