EDC can be used to bioconjugate enzymes to silver nanoparticles with surface hydroxyl groups, but the nanoparticles should ideally have carboxyl groups for effective reaction. If only hydroxyl groups are present, surface modification is necessary to facilitate the conjugation process.

EDC mainly connects carboxyl and amino groups. Although your nanoparticles have hydroxyl groups, you can achieve the connection through some methods. For example, you can first convert the hydroxyl groups into carboxyl groups through some chemical reactions, and then use EDC to connect the enzymes. However, directly using EDC to connect enzymes to nanoparticles with hydroxyl groups may not work well, because the reactivity of EDC with hydroxyl groups is relatively low. So, you might need to first modify the surface of the nanoparticles to increase reaction sites such as carboxyl or amino groups, and then EDC can work better. I hope this helps.

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