Hello! I've just started to work with iPSC cell lines, and I was wondering if there are any checks I could do to ensure that the matrigel coating of my 6 well plates has gone according to plan and can reliably support the growth of my iPSCs.

My workflow is as follows: I quickly thaw a 1ml aliquot of matrigel and transfer it 25ml of ice-cold DMEM-F12 media, using a cold serological pipette. I then store this matrigel dilution at 4°C and use it for a week.

At the moment, I add 1 ml of the matrigel dilution to a single well of a 6-well plate and incubate for about an hour or more before I plate cells on it.

I use the same process for coating wells that I eventually use for differentiating iPSCs into iNeurons, but my cells have been detaching after 9 days of culture.

At the moment, I just look at them under the microscope, and compare them with an uncoated surface, and the coated surface appears a bit more "soft" or "fuzzy", but I was looking for a better pointer/readout.

Thanks in advance!