I work with site-directed mutagenesis, modifying amino acids in ion channel sequences using the splice mutagenesis method. The mutated fragments are then ligated into the full-length channel. However, these fragments are quite large, making it challenging to obtain complete channel constructs.
Michael J. Benedik
Yes it absolutely is possible. People have used T4 ligase to make very large clones (cosmids, BACS etc) and have also ligated tiny DNA sequences such as oligonucleotides.
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Sam Carter
Hi Leticia,
Yes it is possible but indeed it might be a bit tricky. If it's not working try to use the gibson assembly method :)
Have a nice day.
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