24 January 2025 2 5K Report

I have found 3 activity assay kits for microbial transglutaminase as mentioned below:

  • ZediXclusive Microbial Transglutaminase Assay Kit chromogenic, Hydroxamate, Art. No. Z009, kit uses Z-Gln-Gly as the amine acceptor substrate and hydroxylamine as amine donor. In the presence of MTG hydoxylamine is incorporated into Z-Gln-Gly to form Z-glutamylhxdroxamate-glycine which develops a colored complex with iron (III) detectable at 525 nm. This kit is well cited than the other two.
  • Cayman chemical, transglutaminase activity assay kit, Item No. 702250. This kit uses In this assay, transglutaminase transfers an amine group from hydroxylamine (amine donor, Transglutaminase Assay Substrate 2) to a glutamine residue of a peptide substrate (amine acceptor, Transglutaminase Assay Substrate 1). The resulting glutamyl-hydroxamate-containing product forms a chromogenic complex with ferric iron (Fe3+), which is readily detectable at 525 nm. With the Transglutaminase Assay Substrate 1, transglutaminases have a reaction rate that is 16-fold faster when compared to the traditional amine acceptor, Z-Gln-Gly, as the substrate. This kit shows better efficiency than traditional transglutaminase activity assay kit.
  • Sigma-aldrich, transglutaminase activity assay kit, Catalog Number CS1070: The assay is based on transglutaminase catalysis of a covalent bond formation between a free amine group of poly-L-lysine, which is covalently attached to the plate surface, and the gamma-carboxamide group of biotin-TVQQEL-OH substrate present in the Assay Buffer. This reaction immobilizes biotin to the plate surface.

    • Among the three which assay kit will be best suited for microbial transglutaminase?

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