Tips for Generating Caco-2 Spheroids?

25 January 2025 1 5K Report

Hi!

I want to generate basolateral-out spheroids of Caco-2 cells. I have Matrigel at my disposal. I would appreciate any suggestions or protocols.

Thanks!

David Schad

Update: I was able to generate the spheroids. Next, I want to validate apical and basolateral faces using immunofluorescence.

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