we placed plant in a dark conditions (in growth chamber without light) before isolation of mtDNA but still we have little contamination of chloroplast DNA. Is there any other method to control it..
Kindly share which protocol you are using for isolation of mitochondria before isolating mitochondrial DNA. Moreover, how long you kept the plants in dark. Are you separating the mitochondria on percol gradient or a similar one?
Try phenol chloroform step twice to reduce chlorophyll content .
Hi,
Maybe this article is of help for you.
https://www.researchgate.net/publication/312941245_Miniprep_for_chloroplast_and_mitochondria_enrichment_and_DNA_extraction
Good liuck!
Article Miniprep for chloroplast and mitochondria enrichment and DNA...
Hello!! I'd like to ask one thing. I have isolated Chloroplast from leaves with percoll gradient. Chloroplast, observed under a microscope were very beautiful. After I extracted DNA with a DNA extraction kit and amplified with chloroplast genes. I think that in my chloroplast DNA there was nucleus contamination... how can I obtain a Ch DNA without contaminations???
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology