I am working on nitrogen-fixing fresh and marine water cyanobacteria. I want to estimate proline. Whether I should standardize the OD to ensure the exponential phase or the time limit for growth of cyanobacteria to carryout the experiments.
Thank you
OD seems to be a better way. What strain are you working on? Is it filamentous?
Thank you Ankita Kothari. I am working on Nostoc. But how I will standardize that? How to determine specific OD? are there any experiments for find it out? Because, that is growing for more than a month and still it is in log phase. So, time limit is not correct way. Please help.
Thank you
It is unlikely that it can grow in log phase for a month. Have you plotted the growth curve by taking OD measurements at regular intervals?
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