I am doing RNA extraction using Trizol Reagent in vitis. but 260/280 1,00-1,35 and 260/230 0,1-0,3. ı have only chemicals for trizol method. Do ı have to change to method or may ı make to modified to trizol method?
Dear Mehmet KOÇ
Pls. find the attached files
http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-6-27
https://www.researchgate.net/publication/5233050_A_Rapid_and_effective_method_for_RNA_extraction_from_different_tissues_of_grapevine_and_other_woody_plants
https://www.ncbi.nlm.nih.gov/pubmed/18618437
http://link.springer.com/article/10.1023/A:1007433910213
http://onlinelibrary.wiley.com/doi/10.1002/pca.1078/abstract
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article A Rapid and effective method for RNA extraction from differe...
Dear Mehmet KOÇ
what tissue from grapevine are you working on ?
this protocol gives fine results on berries at all devt stage , it was also validated on apples.
best regards
Charles Romieu
Dear Mehmet KOC,
This protocol should be fine for grapevine. The is reagent is useful, You can try again.
Dear My teacher,
Thanks a lot for answers. I understand. ı have to change trizol method. if ı added two steps, first, phenol:clorofom and than clorofom.second, before isoproponal ,50UL sodium asetad .Results will be good. but it wil take a lot time.
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