What is the minimum length of unknown sequence that can be amplified using FirstChoice RLM Race Kit?

I am working on a non-model plant. The sequence of my organism does not exist. I have a RT PCR product that i amplified with primers of the plants within same family. The length of my amplicons...

04 May 2017 4,384 4 View

Can we consider glutamine synthase and glutamine synthetase same?

09 October 2016 4,093 6 View

Can anyone share Direct PCR protocol for plants?

I have to isolate more than 2500 wheat leaf samples for screening. Even we use robotic system, dna isolation is very time consuming and costly. We will use only 2 primer pair for screening. I wish...

09 October 2016 4,723 4 View

How do I discover unknown SNPs with KASPar genotyping system?

We want to discover SNPs in our breeding materials. Cooperation of KASPar and Fluidigm is succesful to identify SNPs, but if we dont know SNPs how we can discover through Kaspar system?...

07 August 2015 7,384 4 View

What's most cost of CRISPR/Cas9 system for plants?

I'm planning to use CRISPR/Cas9 system for my master thesis study. The plant i will work is not certain. It will be tomato or phaseolus. There are a lot kits for other living organisms, but not...

05 June 2015 8,886 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View