Hello dear,

There are many of paid softwares available for the purpose a simple google search will give you many results

However you can use an online software developed by Dr. OP Sheoran a professor at CCSHAU, Hissar. The link is given below

http://14.139.232.166/opstat/default.asp

You can have a try to "NTSYSpc" software which can help you perform multiple tasks for genetic diversity analysis. 

http://www.exetersoftware.com/cat/ntsyspc/ntsyspc.html

Dear Saurav

Could you send me the guideline how to use the softwear