Hi everyone, I'm carrying out a peptide coupling reaction, with the coupling reagents being N,N'-Diisopropylcarbodiimide (DIC) & hydroxybenzotriazole (HOBt). I'm making glycine to couple with 3-chloro-2-methylaniline in the peptide coupling reaction. I have tried dimethylformamide (DMF), ethyl acetate, dichloromethane and acetronitrile, but glycine wouldn't dissolve in them. Kindly advise suitable solvents for the peptide coupling reaction or any chemicals that I should add to help dissolve the glycine. Thank you!
Based on my interpretation of your inquiry, I'd like to know if you employed a base (such as DIPEA or TEA) in the reaction. The reaction would not take place if the base was not there. It's possible that's why your reaction mixture stayed insoluble.
Simply follow the steps below to trigger your reaction.
BOP reagent, TEA & DMF at 70 oC.
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