I am determining the in vitro release profile of citral from chitosan nanoparticles. I will be using the dialysis method using PBS at different ph as the release buffer. During the dialysis, once I put the dialysis membrane containing the sample and buffer into the release buffer in a beaker, do I leave it at room temperature until I take some buffer for analysis or I place it in a shaker and then once my time interval is up, I take the required volume of the release buffer for analysis?
Thank you.
I would put the dialysis membrane in a beaker filled most of the way to the top with the buffer, add a magnetic stir bar, place the beaker on a magnetic stirrer, and set the stirring speed fairly high to make sure the contents of the bag are thoroughly mixed, but not so fast as to endanger a collision between the membrane and the stir bar. The volume of the beaker should be as small as feasible to contain the dialysis membrane while still allowing it to stir, thereby maximizing the concentration of citral in the buffer.
If this would not provide a sufficiently high concentration of citral because of the large volume of buffer, another idea would be to put the dialysis membrane in a sealed tube, such as a 50-ml conical tube with screw cap, filled with buffer and put it on a shaker platform, shaking at the fastest rate you consider to be safe for the dialysis membrane.
A possible problem with this experiment would be if the citral is not soluble in the dialysis buffer.
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