Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

What is the best lighting design for conservation?

In the museums, the sensitive organic materials need to be displayed under 50 lux or less and aways from any UV or IR. LEDs still have a little amount of UV. What is the better option? Is it...

20 January 2021 5,965 3 View

Cryopreservation of cancer cell lines @-80?

07 January 2021 5,410 8 View

Can a researcher depends the conventional PCR as molecular based technique for quantification of STAT3in stead of real time PCR in FFPE tissue ?

The FFPE tissues are relatively difficult to extract the RNA from so, how one can use reverse transcription based PCR

10 December 2020 4,398 6 View

Can anyone help me to solve this problem?

when i did time history anlysis by using sap 2000 , the program showed the first two step only at .02 and .04 . can anyone help me to solve this problem?

27 November 2020 3,810 2 View

Analytical solution of stresses distribution due to tunneling in expansive soil?

I want to know how can I do an analytical solution for stresses distribution due to tunnel excavation in an expansive soil to compare with a numerical simulation. and which method I can use?

22 September 2020 7,602 2 View

Meshing problem in abaqus?

Can any one help me in meshing in ABAQUS, please?

09 September 2020 7,957 7 View

Why halogen species lower the flame temperature?

Chlorine, a component of PVC polymers, acts like a halogen-based flame retardant agent in the event of fire.

07 September 2020 5,450 6 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Why does my H9 IPSC die reasonless after 80% confulency?

Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...

13 June 2020 9,046 3 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

21 December 2019 2,888 14 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

11 December 2019 3,570 6 View

How to eliminate a single component from a medium?

I'm planning to change the components of cell culture media for reprogramming iPSC in order to make it cost-effective for lab research. some research was made in related areas, but my question is...

10 November 2019 9,457 3 View

Why there is no neutrophil progenitors in human as Hoxb8cells in mice which can produce neutrophils?

As question above is there any certain reason that makes human neutrophil progenitors which can produce neutrophils unavailable? I only know HL60 cells and people differentiate neutrophils from...

10 November 2019 7,763 1 View

How can i know -preferably by morphology- that EB are mature and ready for the next step of differentiation?

I am working on differentiating hiPSC into macrophage through EB method, and i wonder how the EB should look like and what are the characteristics before it is cultured with monocytes...

10 November 2019 3,465 2 View