XRF analysis of 45s5 analysis?

I've sent my synthesized 45s5 powder and a commercial 45s5 powder to XRF analysis but in both of them the amount of Na2O is about 15 percent and LOI is around 40 percent and it makes the aount of...

29 January 2021 239 4 View

Sol-gel synthesis of 45S5?

I've synthesized 45S5 bioglass by sol-gel method and my XRD and FTIR results are similar to 45S5 bioglass but my EDX result has differences and instead of Si and Na the amount of Ca is higher....

29 December 2020 4,847 2 View

Sol-gel Synthesis of hydroxyapatite?

I use Ca(NO3)2.4H2O and P2O5 as precursors and Sol-gel method to synthesis my powder but the CA/P ratio is more than stochiometric (167) and my powder is dark. Does anyone know why this happens?

27 December 2020 1,574 5 View

Could anyone help me with this error Exception has occurred: ValueError?

I have downloaded monthly mean wind speed of terraclimate data for 20 years (2000-2019) which have NetCDF format, but unfortunately, each year has a single NetCDF file, and I want to merge them...

25 November 2020 8,477 2 View

Has anyone used Breaks For Additive Season and Trend for decomposing the time series into trend,and season in R package?

12 September 2020 1,513 3 View

How show NDVI MODIS index (MOD13A1) in Arc GIS?This collection of MODIS has calculated NDVI index and i want to show this index in my study area?

This collection of MODIS has 2 options for calculating NDVI index. First, we can calculate it from NDVI formula or we can use NDVI index that MODIS has calculated. I use both of them but they are...

25 August 2020 2,419 3 View

Has anyone used Maximum likelihood or Support vector machine (SVM) for image classification?

Hello I'm working on a project that I want to detect forest and deforestation areas. I need some information about Maximum-likelihood or Support vector machine (SVM) for image classification.

22 August 2020 1,896 3 View

How we can use the Transmisivity of light colors (rtn, gtn, and btn) in simulating a coated glass with red dyes in Radiance?

We want to simulate a red coated glass in Radiance. The UV-VIS spectroscopy analysis was conducted and optical characteristics of the red glass including transmittance and absorbance were...

16 March 2020 3,492 5 View

Model game as a discrete-time Markov chain?

Model game as a discrete-time Markov chain

30 December 2019 5,346 3 View

How can i remove fat from alkaloids?

sometimes i have problem with fat in my extracted alkaloids using ethanol and chloroform for extraction. Also my alkaloids are not well dissolved in acidic water, so acid-base precipitation...

09 December 2018 2,737 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View