How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?
I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?
14 January 2024 2,503 2 View
Can someone provide me with the earthquake repositioning code with Python?
29 September 2023 6,469 3 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
22 July 2023 964 6 View
hello, I have a major question that i would really appreciate if anyone could make me clear. In a project I am going to transfect BMSCs with a plasmid by lipofectamine 2000 reagent. then the...
06 June 2023 9,768 1 View
I want to use an epilepsy database ( CHB-MIT Scalp EEG Database) and the samples have some bipolar channels like FP1-F7, F7-T7, T7-P7, P7-O1, FP1-F3, etc. We can define monopolar channels in...
12 July 2022 3,546 0 View
I dont mean bacterial cellulose(the cellulose which produce by bacteria like G.xylinus ) or the cellulose fibril that present in paper mill activated sludge, i mean a protocol for extracting the...
02 July 2022 6,182 2 View
Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.
07 March 2022 1,689 0 View
Hello everyone I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t...
15 December 2021 3,208 1 View
I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...
23 November 2021 8,236 4 View
I need this card (PDF-49-0442) to analyze my XRD result. Can anyone send it to me?
22 November 2021 4,155 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View