What should be the cell density per ml for RNA quatification?

15 December 2023 1 9K Report

I am trying to learn RNA quantification from BV2 cell using easy blue reagent. but the RNA quantity is very low. may be the cell density per ml is low. i m using 6well plate . i cant find where i m lacking. so my guess is, may b the cell density is low.. need experts help.

Sabine Strehl

As mentioned in the protocol, you should count the cell number and use 3-10 million cells per 1 ml of easy-BLUE.

https://intronbio.com:6001/intronbioen/product/product_view.php?PRDT_ID=25#none

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