A stock solution of 1mg/ml is the same as 1ug/ul. So if you want 5ug, you need 5ul of your stock. Not sure if that's the question you were asking but hopefully that helps.

Hello Aditya Raj

Adding further,

To make 1ug concentration, you will have to take 1ul from 1mg/ml monoclonal antibody solution. Since the volume is too low, there is a high chance of pipetting error.

So, I would suggest that you make a stock solution of 0.1mg/ml by diluting the monoclonal antibody solution (1mg/ml) by 1:10.

So, for 1, 2 and 5ug concentrations you would take 10ul, 20ul and 50ul instead of 1ul, 2ul and 5ul respectively of 0.1mg/ml stock solution.

However, for the remaining concentrations namely, 10, 20, 40 and 80ug you would take 10ul, 20ul, 40ul and 80ul respectively of the original 1mg/ml monoclonal antibody solution.

Best.