Best qualitative method for sequential explanatory dissertation?

Hello, I am conducting a dissertation using a sequential explanatory design. The order of the design is quantitative, quantitative, qualitative with more or less equal emphasis on each phase,...

14 March 2020 8,153 3 View

Can I use touchdown PCR for a 16S microbiome sequencing study?

Hi everyone. Just looking for some thoughts on this. My thinking is that I shouldn't use touchdown PCR to amplify 16S regions in this case because it will cause bias. The primers will already...

09 January 2020 8,577 1 View

Is salt to generate chlorine for swimming pools toxic?

I am trying to aquire recent research, that either confirms or disproves, that salt used to generate chlorine for swimming pools is more toxic to humans than standard clorine. Thanks! David

27 May 2019 8,034 3 View

Journal suggestions needed for parasite prevalence in Sargassum-associated shrimp?

Long story short, my MS thesis looked at Sargassum associated macrofauna. My fellow collaborators and I are currently writing everything up for publication as part of larger study but during the...

28 August 2018 793 1 View

How to get individual pixel values from line ROI's in ENVI?

I have 23 lines in my ROI, I want to extract the values for each individual line. When I run basic statistics it treats all lines in the ROI as one so I only get one line of statistics. I need all...

15 May 2018 3,736 2 View

What is the best strategy to introduce a HA-tag?

Dear All, I have a protein of interest that is fused to GFP at its C-terminus. I would like to add a HA tag at the N-terminus, and was wondering what strategy to use. Should I just introduce the...

08 September 2016 4,201 5 View

What are the most appropriate statistics to use for my experimental design?

11 May 2016 7,914 3 View

How do I stop RIPA-insoluble, Urea-soluble protein fraction from precipitating in Western blot wells?

18 November 2015 2,156 7 View

Which fluorophores are stable at pH 5?

Does anyone know which fluorophores are stable at low pH? I'm doing ISH combined with cell surface staining and then analysing the cells by flow cytometry. Sadly, the ISH needs to be carried out...

21 February 2012 6,730 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View