List python packages for Metabolomic data mainly of GCMS?

Hi, I want to know what are the python packages available for metabolomic data analysis. I have heard that R programming is best for statistical analysis and has some good packages in it. But I...

01 November 2020 3,071 3 View

Which language should I learn either matlab Or python?

I'm a Mtech student and want to get involved in #angiogenesis field. And now, I want to learn a programing language that suits my field most. Please can anyone suggest me which language is best...

04 October 2020 3,638 15 View

What are the techniques to be used In implementing fast routing in SDN?

I am doing research on the topic "Implementing Fast Routing in SDN". I need help to get related research papers so I can continue my research work efficiently.

23 September 2020 8,327 3 View

Which NCD is likely to increase the most after Covid-19 pandemic is over?

We have seen that the mortality among the Covid patients suffering from NCDs is very high. So we need to protect NCDs patients from covid infection.But a rise is expected among NCDs after the...

23 September 2020 4,080 4 View

In substitutional solid solutions, do the substituting atoms form chemical bonds as they replace the atoms? How are they replacing the existing atom?

I do not understand how atoms replacing the base metal in substitutional alloys actually replace the base metal atoms and not form bonds.

20 September 2020 433 3 View

What are the data that give storm/solar flare characterization for the region?

I have been using Dst & Kp for storm and solar activity identification. However, I understand these indices gives global information. I am interested in knowing the storm/flare condition in a...

03 September 2020 9,120 3 View

Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

We know there is a substantial reduction in tobacco use in India as per GATS-2. Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

25 August 2020 9,254 1 View

How to find the significant difference between three groups through confidence interval in ANOVA test?

Dear researchers, KIndly clarify me that, we are doing One way ANOVA test to find the difference between three groups. My question is.... How can we analyze the significance between the groups...

07 June 2020 2,576 4 View

Would a light smearing of the insides of nostrils with oil possessing anti-microbial properties like coconut oil reduce COVID-19 virus attachment?

Coconut oil is known to have anti-microbial properties. Would it's smearing of insides of nostrils and hands and other exposed body parts help in reducing the spread of COVID-19 virus...

26 March 2020 9,055 14 View

Topics that must be included while initiating AI studies. Kindly share your ideas to device a perfect learning path ?

There are several books, publications, syllabus and courses but what could be possibly a holistic syllabus with a connection among topics for better learning and understanding

06 February 2020 355 3 View

Tricine SDS-PAGE advice?

15 February 2021 3,497 3 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

How to optimize cell suspension protocol for flow in LS-180 cell line?

Hi, I am optimizing a flow protocol for LS-180 cell line, which can not be trypsinized. First experiment wend suboptimal due to detritus and doublets Any trips or tricks for making this cell...

06 January 2021 6,117 2 View

What are these black and white spheres in T25 flask?

I just thawed my 4T1 cancer cells and 24 hours after seeding the cells in T25 flask there were large black and white spheres (approximately 1000times bigger than my cells) in my cell...

18 December 2020 3,302 3 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Any expert on Calu-3 cell culture?

I just started working months ago with this peculiar cell line and I still cannot figure out the doubling time or passaging. Some times take 72h to see double amount of seeded cells, some times...

23 October 2020 6,151 2 View