Hi everyone!

my protein crystals like as follow(picture1),aithough they can grow bigger,but seem not to be a single crystal, and without good three-dimensional forms. how should i optimize them? could anyone give me some advices?

thanks!

my crystallizton condition are as follow:

protein concentration :10mg/ml

drop ration: 1:1

temp: 4 ℃

crystallization buffer: 0.1M Tris-HCl,0.2M(NH4)2SO4、25%PEG 3350、1mM NiCl2

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