Hi everyone!
my protein crystals like as follow(picture1),aithough they can grow bigger,but seem not to be a single crystal, and without good three-dimensional forms. how should i optimize them? could anyone give me some advices?
thanks!
my crystallizton condition are as follow:
protein concentration :10mg/ml
drop ration: 1:1
temp: 4 ℃
crystallization buffer: 0.1M Tris-HCl,0.2M(NH4)2SO4、25%PEG 3350、1mM NiCl2