To measure GLP-1 concentration in blood we must add DPP-1 inhibitor to the blood sample. I tried to dissolve linagliptin or alogliptin in DMSO but this caused severe hemolysis.
Can anyone help me how to add DPP4 inhibitors to blood samples?
Follow the following steps
Select a DPP-4 Inhibitor: Commonly used DPP-4 inhibitors include Sitagliptin, Vildagliptin, or Linagliptin. Choose one based on availability and compatibility with your analysis method.Prepare Stock Solution: Dissolve the chosen DPP-4 inhibitor in an appropriate solvent to create a stock solution. Ensure the concentration is suitable for your experiment.Add DPP-4 Inhibitor to Blood Samples: Mix the stock solution with the blood samples immediately after collection. The concentration of the DPP-4 inhibitor should be sufficient to inhibit the enzymatic activity effectively.Mix Gently: Ensure thorough mixing of the DPP-4 inhibitor with the blood samples to distribute it uniformly.Incubation: Incubate the samples for a specified time at an appropriate temperature to allow the inhibitor to act on DPP-4 and inhibit GLP-1 degradation.Centrifugation: Centrifuge the samples to separate plasma or serum from cellular components.Store Properly: Store the treated samples at the recommended temperature and conditions to maintain the stability of GLP-1 until analysis.
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