Doing an in vitro study with nanostructured lipid carriers with vitamin D and with just vitamin D alone, and just trying to figure out the optimal incubation time so I don't stop it too late having saturated the cells and wind up seeing no difference.
Xiaoyun Li
Hi, may be you should do some blood concentration monitoring experiments
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Shanshan Li
You may have a look at the literature below for nanostructured lipid carriers with vitamin D
Article Chitosan coated nanostructured lipid carriers (NLCs) for loa...
For vitamin D alone incubated in Caco-2 cells, I recommend 10 nM 1, 25D3 for 24 hours as a physiological condition, or you can try 100 nM 1, 25D3 for 24 hours to check the maximal changes.
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