25 February 2020 8 4K Report

I use the same primer set and same conditions. The first PCR product from cDNA gave about 800 bp. Reamplification of the template gave 700 bp. A second amplification gave 600 bp.

Interestingly, I did an amplification of the original cDNA today and I got 600 bp. Any ideas whats happening? All this is happening within a week with products stored at -20 deg celsuis.

Thanks

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