Can intracellular proteins fused to GFP be detected by flow cytometry?

20 January 2024 1 900 Report

If cells were transfected with a plasmid containing a gene of interesed (the resulting protein is intracellular) fused to GFP, would the GFP signal be detected by flow cytometry? Or can we only detect those proteins on the cell surface?

Sabine Strehl

Yes, the GFP signal can be detected by flow cytometry no matter if intracellular or on the surface.

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Science method

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