Hello, all
I am currently extracting LPS from E. coli MG1655 and its mutant strains.
I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see the ladder pattern of LPS. What I can see clear is the fat band at the bottom of SDS-PAGE (probably lipid A part?)
Can anyone give me suggestions? Is culture condition or pH important? Is LPS degraded during prep?
I haven't done LPS extraction myself, but it has been done in our lab (PMID: 31115938). We used a BocaScientific LPS extraction Kit (Cat#: 17141) and then a Bio-Rad Silver Stain Kit (Cat#: 1610449) for visualization on SDS-PAGE. If I remember well, the extraction kit was working really well and the straining requiered more troubleshooting than the extraction. Of course, different bacterial strain will give different LPS yield, so I cannot say for E. coli MG1655.
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