Hello, sporulation of the Saccharomyces cerevisiae is a response to nutrient depletion that allows a single diploid cell to give rise to four stress-resistant haploid spores. The formation of these spores requires a coordinated reorganization of cellular architecture. Keep plate in refrigerator 2-8*C for promotion of sporulation.

Good luck.

Hi Marco,

I have sporulated yeast cells before by plating on a nutrient-poor agar from fresh overnight cultures. I suppose you could use the liquid media form in a 96 well plate to sporulate the strains there. if you do a literature search you should be able to find a good media recipe.

What will you ultimately measure? I think if you outlined your goals in a little more detail, the community may be more suited to help with your question.

Check out papers working with the Synthetic Genetic Array method, it uses high-throughput sporulation and has specific media for that. Article Synthetic Genetic Array (SGA) Analysis in Saccharomyces cere...