Hello,
From my understanding, there are two ways to add polyA tails to RNA.
1) with polyA polymerase (PAP) and ATP to RNA or 2) with terminal transferase (TdT) plus dATP and ddATP to ssDNA (cDNA?)
However, I am wondering what is the pro/con for each method. Currently, the way I see it, is that it may be better to use the TdT method on cDNA which would be more stable than mRNA.
Does anyone have any ideas on this or why the PAP method would be better than TdT?
Thanks!
Morgan
Ajay Saini
Hi
As you mentioned, PAP is for adding poly A to RNA molecules (where PolyA tail is not present) and TdT adds A-tail to the cDNA but not RNA molecules. These two are different reactions. Both are fine and if done with proper care PAP will work quite efficiently
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