Hello everyone,
For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I have only ever grown E.coli before so I'm not confident with the lactobacillus growth conditions.
Can you please provide me with some information about growing different lactobacillus species? Preferably aerobically as it will be more difficult for me to access an anaerobic chamber...
I was thinking of ordering some cells from ATCC (https://www.lgcstandards-atcc.org/products/all/23272.aspx#generalinformation). Is it possible to use the same agar and LB broth recipe as I'd use for e.coli or does it need to be altered for lactobacilli?
Any advice would be much appreciated. (As detailed as possible).
Dear Kate
All species of Lactobacillus can grow in aerobic condition. All species of Lactobacillus found in GI of human and animals. They are growing in aerobic condition.
Hi. In liquid condition I presume ?
I think the MRS medium is the major one, and its composition is :
https://en.wikipedia.org/wiki/MRS_agar#:~:text=De%20Man%2C%20Rogosa%20and%20Sharpe%20agar%2C%20often%20abbreviated,de%20Man%2C%20Morrison%20Rogosa%20and%20Margaret%20Elisabeth%20Sharpe.
A comparative of growth medium can be found here:
Article GROWTH OF LACTOBACILLUS SPECIES ON DIFFERENT MEDIA
Or, for semi-solid medium, I google found this publication for optimized growth condition in semi solid : https://pubmed.ncbi.nlm.nih.gov/33260858/
Best,
The best medium used in growing LAB is MRS, broth is recommended. Most LAB are mesophiles, can be isolated from dairy products or infant stool
Dear Kate,
According to my experience in working with different species of lactobacilli, growth is better in conditions of weaker aeration. In general, lactobacilli are aerotolerant anaerobes and microaerophilic. In a situation when I did not have optimal conditions, I overcame that problem by filling the Erlenmeyer with a larger volume of nutrient medium.
I used MRS agar and MRS broth to cultivate lactobacilli
Dear Kate;
Some research articles indicate that Lactobacilli can grow in anaerobic conditions. They can also grow in microaerophilic conditions. Currently, I study the activity of these bacteria against some foodborne pathogens. I have been trying the MRS agar with a pour-plate method and, then a thin layer pouring on the same agar after it becomes solidified, considering them as microaerophilic.
Dear Kate,
According to my experience,Rogosa medium can also be used for growth of lactic acid bacteria and it also gives better results.lactobacillus can grow in microaerophilic condition. Firstly enriched the bacteria in broth and then plated on agar medium.
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