To develop a neonatal intestinal organoid, what are the appropriate ages of the mice to be dissected to compare a neonatal mouse to an adult mouse?
We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...
19 February 2021 8,478 2 View
actually, I am degrading pesticides by using inorganic composites the pH of the pesticide in distilled water is neutral the pH of nanocomposite is also neutral then why maximum degradation takes...
23 January 2021 8,775 3 View
Usually, in COMSOL MultiPhysics, there is an option to choose the parameter value from material properties. For example, for choosing Young's modulus value E in the solid mechanics module, one can...
07 January 2021 8,577 8 View
Concrete block bricks companies are marketing that their products are eco-friendly, as they are not emitting carbon dioxide and not using soil. At that viewpoint, they are right. But,in their...
04 January 2021 5,641 8 View
" Psychosocial Issues among Breast Cancer Patients in Asian Developing Countries" is my article but another Sajid Iqbal has added it to his profile on research gate, how can we let him know his...
29 December 2020 5,736 4 View
Which specific environmental problems can be accessed or solved by environmental modeling?
25 December 2020 1,312 0 View
Assessing professionalism is very challenging these days when most of assessment is online . We can declare students proficient un till unless we assess professionalism.
21 December 2020 2,467 3 View
I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?
18 December 2020 7,575 1 View
For image processing, I am using ImageJ. I need to separate the features from the background and calculate pixel intensity of features and background. Can anybody help me in this? Thanks
09 December 2020 7,472 9 View
Hi everyone, I am a student trying to quantify the number of cells in an image using ImageJ. Is there a way to bring all my images from a set of experiments to a set intensity? For example, if...
05 December 2020 7,088 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View