Anaerobic microorganisms are very common in periodontal diseases especially periodontitis. My concern is how to isolate those organisms and transport it to the microbiological lab in a easy way.
Hi Rachita. I don't have experience with periodontal bacteria, but a stab culture is typically the easiest way to isolate/culture anaerobic bacteria. There are plenty of resources that can be found by googling "stab culture". Hope this helps.
Peridontitis dental caries anaerobic culture studies of advanced disease provided a comperhensive analysis of microbiota of these infections more details in the folloeing
Ref,Journal of Oral Biosciences.Vol.57.Issue 1.February2015 page 18_26
You need transport media, samples should be placed in fluid thioglycollate medium in a test tube and transport to the laboratory, and processed immediately.
Kindly check the following RG link:
Article Microbial Flora in Chronic Periodontitis: Study at a Tertiar...
In it, it has been explained in detail how to transfer and deal with samples and how to deal with aerobic and anaerobic bacteria.
I think the reference illustrate every thing fot such fastidious anaerob bacteria.
Dear all :The journal of Oral Biosciences is specfic one dealing with oral microbiotas where anaerobic culture highlighted with selective media illustrated far away from traditional transport media ,samples for culture were taken and put in PRAS Ringers salts solutions,processed and incubated anaerobically as far as previous in advanced and initial periodontitis,while samples from children caries and caries_free control children were cultured on a complex blood agar to facilitate growth of fastidious anaerobes.please who recommend must consider subject specificity.
Read well the attached reference .
Best regards
https://www.sciencedirect.com/science/article/pii/S1349007914000917
Dr.Rachita Mustilwar,please your question
focused on two points:a_Anaerobic bacteria related with b_Periodontitis.
I gave study showed PARS Ringers salts solutions as trsnsport media ,processed and incubated anaerobically and prcessed for anaerobic bacteria while cases for children cultured on blood agar anaerobically for isolation of Fastediou anaerob bacteria.please give your laboratory evaluations between these selective specific methods and survay study using thioglucolate as transport media,thanks for such illustration.
Best regards
Hi. I hope the following article could help you:
https://sjomr.org.in/index.php/SJOMR/article/download/93/93
I have used “Weinberg tubes” to get isolated round (yes, three-dimensional) black colonies of sulfate-reducing bacteria, through serial dilution in an agar medium supplemented with ammonium iron (II) sulfate (Mohr's Salt).
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